Anti-MAPK6/ERK3 antibody [EP1720Y] ab53277 from Abcam

Product Specs
ItemAnti-MAPK6/ERK3 antibody [EP1720Y]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab53277
Antibody TypePrimary
ClonalityMonoclonal
HostRabbit
ApplicationsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Quantity10 UL, 40 UL, 100 UL
CloneEP1720Y
IsotypeIgG
Conjugate/TagUnconjugated
Concentration2.228 mg/ml
Gene NameMAPK6
ValidationKnockOut Validated
ReactivityHuman, Mouse, Rat
TargetMAPK6 / ERK-3

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Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) ERK3/MAPK6 dictates CDC42/RAC1 activity and ARP2/3-dependent actin polymerizationeLifeApril 14, 2023Katarzyna Bogucka-Janczi, Gregory Harms, Marie-May May-Coissieux, Mohamed Bentires-Alj, Bernd Thiede, Krishnaraj Rajalingamcells were incubated in 1:250 dilution of anti-ERK3 antibody (mouse Cat# MAB3196, R&D or rabbit Cat# ab53277, Abcam) in blocking solution for 1 hr at RT. For the ARP3 and CDC42 staining, the anti-ARP3 antibody

(2) Increased methylation of ZNF671 suppresses tumor progression by promoting MAPK6 transcription in laryngeal carcinomaInternational journal of biological sciencesJanuary 1, 2023Zhang W, Liu T, Li X, Li T, Ma X, Zhao D, Zhao X.follows: ZNF671 (Sigma-Aldrich, Shanghai, China; HPA046099, 1:100), MAPK6 (Abcam, Cambridge, MA, USA; ab53277, 1:100), Ki67 (Abcam, ab15580, 1:100), E-cadherin (Abcam, ab76055, 1:100), N-cadherin (Abcam, ab76011,

(3) On the Therapeutic Potential of ERK4 in Triple-Negative Breast CancerCancers (Basel)December 21, 2022Fadia Boudghene-Stambouli, Mathilde Soulez, Natalia Ronkina, Anneke Dörrie, Alexey Kotlyarov, Ole-Morten Seternes, Matthias Gaestel, Sylvain Melocheobtained from the following suppliers: anti-ERK4 (1/1000; #AP7298b) from Abcepta; anti-ERK3 (1/1000; #ab53277) and anti-phospho-MK5(Thr182) (1/1000; #ab138668) from Abcam; anti-HA (1/5000; #901501) from BioLegend;

(4) miR-128-3p inhibits the inflammation by targeting MAPK6 in penicillin-induced astrocytesNeuroReportDecember 14, 2022Yuejiu Pang, Dingzhen Luo, Shuhua Wang(ab200478; RRID:AB_2888939; 1:1,000; Abcam), TNF-α (ab205587; RRID:AB_2889389; 1:1,000; Abcam), MAPK6 (ab53277; RRID:AB_2140288; 1:1,000; Abcam) and GAPDH (ab181602; RRID:AB_2630358; 1:1,000; Abcam) at 4°C overnight;

(5) Systematic discovery of mutation-directed neo-protein-protein interactions in cancerCellMay 26, 2022Mo X, Niu Q, Ivanov AA, Tsang YH, Tang C, Shu C, Li Q, Qian K, Wahafu A, Doyle SP, et al.antibody,Cell signaling,Cat# 8943; RRID:AB_11024092 Rabbit monoclonal anti-MAPK6 antibody,Abcam,Cat# ab53277;RRID:AB_2140288 Rabbit polyclonal anti-ITPKB antibody,Proteintech,Cat# 12816-1-AP; RRID:AB_2129846 Rabbit

(6) Paclitaxel binds and activates C5aR1: A new potential therapeutic target for the prevention of chemotherapy-induced peripheral neuropathy and hypersensitivity reactionsCell death & diseaseMay 25, 2022Brandolini L, d'Angelo M, Novelli R, Castelli V, Giorgio C, Sirico A, Cocchiaro P, D'Egidio F, Benedetti E, Cristiano C, et al.Cell Signaling), rabbit anti-p-MAPK6/ERK3 1:1000 (S189, ab74032, Abcam), rabbit anti-MAPK6/ERK3 1:1000 (ab53277, Abcam). As secondary antibodies, peroxidase-conjugated anti-rabbit or anti-mouse IgG (1:30000; Invitrogen,

(7) Conditional ERK3 overexpression cooperates with PTEN deletion to promote lung adenocarcinoma formation in miceMolecular OncologyMarch 1, 2022Sreeram Vallabhaneni, Jian Liu, Marion Morel, Jixin Wang, Francesco J. DeMayo, Weiwen Longhematoxylin. The primary antibodies used for immunohistochemistry are anti‐ERK3 (1 : 50 dilution, Abcam #ab53277, Waltham, MA, USA), anti‐TTF1 (1 : 1000 dilution, DAKO #M3575, Santa Clara, CA, USA), anti‐P63 (1 : 50

(8) Rab31 promotes the invasion and metastasis of cervical cancer cells by inhibiting MAPK6 degradationInternational journal of biological sciencesJanuary 1, 2022Huang Y, Liu R, Han X, Hou X, Tian Y, Zhang W.Sample Kit (1:1000; 9782; Cell Signaling), Lamin A/C (1:1000; 4777; Cell Signaling), MAPK6 (1:2000; ab53277; Abcam), MMP2 (1:1000; 40994; Cell Signaling), MMP9 (1:1000; 13667; Cell Signaling), p53 (1:2000; 10442-1-AP;

(9) ERK3 is transcriptionally upregulated by ∆Np63α and mediates the role of ∆Np63α in suppressing cell migration in non-melanoma skin cancersBMC CancerDecember 1, 2021Eid S. Alshammari, Amjad A. Aljagthmi, Andrew J. Stacy, Mike Bottomley, H. Nicholas Shamma, Madhavi P. Kadakia, Weiwen Longantibodies were used in immunoblotting: Mouse monoclonal anti-pan-p63 (4A4), rabbit monoclonal anti-ERK3 (ab53277, 1:1000, Abcam), rabbit polyclonal anti-pRac1 S71 (sc-12924, 1:500, Santa Cruz), mouse monoclonal anti-Rac1(ab33186,

(10) MAPK6-AKT signaling promotes tumor growth and resistance to mTOR kinase blockadeScience AdvancesNovember 12, 2021Qinbo Cai, Wolong Zhou, Wei Wang, Bingning Dong, Dong Han, Tao Shen, Chad J. Creighton, David D. Moore, Feng Yangand MK5 (#7419) were from Cell Signaling Technology (CST). Other antibodies used include anti-MAPK6 (#ab53277, Abcam), anti–β-Actin (#AC026, Abclonal), anti-FLAG (#200474, Agilent), and anti-MAPK4 (#AP7298b, Abcepta). In vitro

(11) Macrophage polarization-associated lnc-Ma301 interacts with caprin-1 to inhibit hepatocellular carcinoma metastasis through the Akt/Erk1 pathwayCancer cell internationalAugust 10, 2021Luo HL, Luo T, Liu JJ, Wu FX, Bai T, Ou C, Chen J, Li LQ, Zhong JH.(1:1000), Ki67 (27309-1-AP, Proteintech) (1:1000), anti-AKT1 (ab179463, Abcam) (1:1000), anti-ERK-3 (ab53277, Abcam) (1:1000), caprin-1 (ab241071, Abcam) (1:1000), and GAPDH (ab9484, Abcam) (1:5000) were used

(12) Atorvastatin Upregulates microRNA-186 and Inhibits the TLR4-Mediated MAPKs/NF-κB Pathway to Relieve Steroid-Induced Avascular Necrosis of the Femoral HeadFrontiers in PharmacologyApril 16, 2021Yusong Zhang, Limin Ma, Erhai Lu, Wenhua Huangab192890), Beclin-1 (1:1,000, ab210498), TLR4 (1:1,000, ab95562), p38MAPK (1:1,000, ab31828), ERK (1:1,000, ab53277), JNK (1:1,000, ab124956), NF-κB (1:1,000, ab16502), and Tubulin (1:5,000, ab7291) (all from Abcam Inc.,

(13) Down-Regulation of AHNAK2 Inhibits Cell Proliferation, Migration and Invasion Through Inactivating the MAPK Pathway in Lung AdenocarcinomaTechnology in Cancer Research & TreatmentOctober 1, 2020Dong-Wei Wang, Hai-Zheng Zheng, Na Cha, Xiao-Jie Zhang, Min Zheng, Ming-Ming Chen, Li-Xiang TianAHNAK2 (ab70053), Bcl-2 (ab32124), Bax (ab32503), p-MEK (ab214445), MEK (ab33918), p-ERK (ab73209), ERK (ab53277), p-P90RSK (ab32203) and P90RSK (ab32114), as well as secondary antibody (1:10,000; Abcam, Cambridge,

(14) MicroRNA‑490‑3p suppresses the proliferation and invasion of hepatocellular carcinoma cells via targeting TMOD3Oncology LettersAugust 6, 2020Honghua Wang, Guobao Yang, Yang Yu, Peibing Gu Peibing Guno. ab8805), anti-p38 (dilution, 1:1,000; cat. no. ab31828), anti-ERK (dilution, 1:1,000; cat. no. ab53277) and anti-β-actin (dilution, 1:1,000; cat. no. ab8226). All the antibodies were purchased from Abcam.

(15) Circular RNA MAPK4 (circ-MAPK4) inhibits cell apoptosis via MAPK signaling pathway by sponging miR-125a-3p in gliomasMolecular cancerJanuary 28, 2020He J, Huang Z, He M, Liao J, Zhang Q, Wang S, Xie L, Ouyang L, Koeffler HP, Yin D, et al.(ab170099, Abcam), anti-p38 (phospho T180 + Y182) antibody (ab4822, Abcam), anti-MAPK6/ERK-3 [EP1720Y] (ab53277, Abcam), anti-ERK1 (phospho T202) + ERK2 (phospho T185) [EPR19401] (ab201015, Abcam), anti-JNK (66210–1-Ig,

(16) The atypical MAPK ERK3 potently suppresses melanoma cell growth and invasivenessJournal of Cellular PhysiologyAugust 1, 2019Minyi Chen, Amanda K. Myers, Michael P. Markey, Weiwen Longprimary antibodies were used: anti-BRAF (sc-5284; Santa Cruz Biotechnology, Dallas, TX), anti-ERK3 (ab53277; Abcam, Cambridge, UK), anti-pERK1/2 (4370; Cell Signaling Technology, Danvers, MA), anti-Rictor (A300-459A-M;

(17) Germ Line Deletion Reveals a Nonessential Role of Atypical Mitogen-Activated Protein Kinase 6/Extracellular Signal-Regulated Kinase 3Molecular and Cellular BiologyMarch 15, 2019N. Ronkina et al.Hybond ECL nitrocellulose membranes (GE Healthcare). Primary antibodies used were anti-ERK3 (EP1720Y; ab53277) from Abcam, MK5 (HPA015515) from Atlas antibody, and GAPDH from Millipore. Secondary horseradish peroxidase-conjugated

(18) L290P/V mutations increase ERK3’s cytoplasmic localization and migration/invasion-promoting capability in cancer cellsScientific ReportsDecember 1, 2017Hadel Alsaran, Lobna Elkhadragy, Astha Shakya, Weiwen Longpreviously7,12. The following primary antibodies were used in Western blotting: anti-ERK3 (Abcam, Cat# ab53277), anti-p-ERK3 (S189) (generated for our lab by the monoclonal antibody core facility at Baylor School

(19) Metformin potentiates the effect of arsenic trioxide suppressing intrahepatic cholangiocarcinoma: roles of p38 MAPK, ERK3, and mTORC1Journal of Hematology & OncologyDecember 1, 2017Sunbin Ling et al.MAPK(Thr180/Tyr182, #4511), and Ki-67 (#9449) were from Cell Signaling Technology, Inc. (Danvers, MA, USA). ERK3(ab53277) was from Abcam (Cambridge, MA, USA). Goat anti-rabbit and goat anti-mouse IgG peroxidase-conjugated

(20) Long non-coding RNA PTENP1 inhibits proliferation and migration of breast cancer cells via AKT and MAPK signaling pathwaysOncology LettersOctober 1, 2017Sheng Chen, Ye Wang, Jian-Hua Zhang, Qi-Jun Xia, Qiang Sun, Zhen-Kai Li, Jian-Guo Zhang, Mao-Sheng Tang, Mao-Sheng Dong

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Anti-MAPK6/ERK3 antibody [EP1720Y] from Abcam

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Anti-MAPK6/ERK3 antibody [EP1720Y] from Abcam

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